- Open the swab sachet that will be used to collect the specimen.
- Use a swab to collect a specimen from the nose or throat according to the type of swab used.
- Insert the swab into the tube containing the VTM fluid and then break the swab.
- Tighten the tube cap and shake it 5 times. Then add the patient ID.
- ITM tubes containing sample specimens can be sent to the laboratory for PCR process at room temperature (0°C – 35°C)
ITM / VTM Test Schematic Diagram
A. The collection of specimens and the deposition of potential SARS-CoV-2 virus particles can be carried out Active or Inactive.
B. Active Transport Media (VTM) must be extracted, while Inactive Transport Media (ITM) should not be extracted because it uses a lysis buffer, a DNA isolation method.
C. Transfer to PCR plate format where cDNA is synthesized and detected by RT PCR
