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Panduan Penggunaan ITM

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  1. Buka sachet swab yang akan digunakan untuk mengambil spesimen.
  2. Gunakan swab untuk mengambil spesimen pada hidung atau tenggorokan sesuai jenis swab yang digunakan.
  3. Masukkan swab ke dalam tabung yang berisi cairan VTM kemudian patahkan swab.
  4. Kencangkan tutup tube lalu dikocok 5x. Kemudian beri identitas pasien.
  5. Tabung ITM yang berisi sample spesimen dapat dikirim ke laboratorium untukproses PCR dengan suhu ruang (0°C – 35°C)

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Diagram Skematik Pengujian ITM / VTM

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A. Pengumpulan specimen dan pengendapan partikel virus SARS-CoV-2 yang potensial dapat dilakukan Active atau Inactive
B. Active Transport Media (VTM) harus dilakukan ekstraksi, sementara Inactive Transport Media (ITM) tidak harus dilakukan ekstraksi karena sudah menggunakan lysis buffer, metode isolasi DNA
C. Transfer ke format plate PCR yang mana cDNA disintesis dan deteksi oleh RT-PCR

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